Bacterial luciferase subunits are synthesized in equal quantities.
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چکیده
منابع مشابه
Individual subunits of bacterial luciferase are molten globules and interact with molecular chaperones.
We have studied the assembly of a large heterodimeric protein, bacterial luciferase, by mixing purified subunits expressed separately in bacteria. The individual subunits alpha and beta contain much (66% and 50%, respectively) of the alpha-helix content of the native heterodimer as measured by circular dichroism, yet the alpha subunit lacks observable tertiary structure as measured by NMR. Thes...
متن کاملIn vitro synthesis of subunits of bacterial luciferase in an Escherichia coli system.
RNA extracted from the luminescent bacterium Vibrio harveyi was translated in an Escherichia coli system. RNA from highly luminescent cells produced both alpha and beta subunits of luciferase in vitro, as confirmed by immunoprecipitation and partial proteolysis. RNA from cells before induction of luminescence did not direct luciferase synthesis.
متن کاملStructure of bacterial luciferase.
The generation of light by living organisms such as fireflies, glow-worms, mushrooms, fish, or bacteria growing on decaying materials has been a subject of fascination throughout the ages, partly because it occurs without the need for high temperatures. The chemistry behind the numerous bioluminescent systems is quite varied, and the enzymes that catalyze the reactions, the luciferases, are a l...
متن کاملA cyanide-aldehyde complex inhibits bacterial luciferase.
Cyanide at high (millimolar) concentrations inhibited in the in vitro Vibrio harveyi luciferase reaction. Cyanide reacted with free aldehyde to form an inhibitor. Inhibitor formation was accelerated by alkaline conditions and bovine serum albumin.
متن کاملCrystalline Bacterial Luciferase from Photoberterium Fischeri.
13 Yphantis, D. A., Biochemistry, 3, 297 (1964). We are indebted to Dr. Vern Schumaker for the determination of the molecular weights by this method. 14 Arnaud, C., C. Anast, and A. Tenenhouse, in preparation. 15 Rasmussen, H., C. Arnaud, and C. Hawker, Science, 144, 1019 (1964). 16 Moore, S., personal communication. We are indebted to Dr. Moore for several helpful discussions during the course...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1977
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)32789-8